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Submission Advice

Eyes (Whole Globes)

Please fill in the submission form as fully as possible; a summary of the clinical history is preferable to a printout of clinical notes.
  • indicate the eye affected, and if the condition is uni- or bilateral
  • use diagrams provided to mark areas / lesions of interest
  • clinical history is vital to interpretation of histological findings
  • please indicate if an intraocular lens implant (IOL) is present, and if its is a hard or soft (foldable implant)

Tissue Preparation

  • trim away excess tissues for optimal fixation, unless involved in disease process (nictitans will not interfere with fixation)
  • please leave enucleated globes intact (incision causes collapse and distortion, hampering gross evaluation)
  • orientation is still possible without lids, however location of lesions of interest may be marked with a suture if desired

Front view of the eye
Caudal view of the eye
Front and caudal views of eye correctly submitted.
Trimming away excess tissue allows optimal fixation.

General Submission Advice

  • handle tissues gently to avoid artifacts (be aware that cautery will cause artifactual changes)
  • place tissue into fixative rapidly to avoid desiccation
  • always use a suitable volume of fixative, a 1:10 ratio of tissue sample to neutral buffered formalin
  • use a suitable size of submission container (please DO NOT cram tissue into a narrow-necked container, as the container may need to be broken to retrieve the biopsy once it is fixed and no longer flexible)
  • label the submission container with the animal and owners name, and site of biopsy
  • please do not use sellotape to seal the container  (Parafilm M Barrier film is acceptable if additional sealing is required)
  • fill in the submission form with as much clinical detail as possible (please don't forget to add your practice details - it happens!)

Sample Packing Instructions

  • use a leakproof sample container
  • label the container with animal, owner and practice details, and biopsy site if more than one container is sent for a particular case
  • wrap the container in sufficient absorbent material (i.e. cotton wool) to absorb all liquid in the event of breakage or a leak in transit
  • pack the container in a leakproof outer container (Ziploc bag)
  • place in a padded envelope, add practice (sender) details to reverse
  • apply a laboratory address label identifying the item as a Category C / Exempt pathological sample to conform to postage safety regulations (These are available to download from the Submission Forms page)